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Using 25% of its maximum HP, the user creates a substitute with 1 HP more than the amount of HP lost by the user. If the user's maximum HP is 3 or less, it will not lose any HP when the substitute is made. A substitute will have the same current type and current stats as the Pokémon that created it.

Once created, all stat modifying attacks and side effects of attacks used by the opponent against the user will fail, though all current stat modifiers will remain in effect and any stat modifiers used by the user will also be applied to the substitute. The substitute will break when the damage the substitute has taken is equal to or greater than the HP used to make it. Until it breaks, a substitute will absorb all damage done by the opponent (even if the damage done exceeds the remaining HP of the substitute), but will not reduce the actual amount of damage that the opponent's attacks do. A one-hit KO move, if it hits, will always break a substitute.

Substitute will not protect the user from self-inflicted status conditions, but it will protect it from any status condition generated by an opponent's move and damage due to those status conditions.

Substitute will not alter the execution of Bide, Counter, Disable, Haze, Leech Seed, Super Fang, Transform, or partial trapping moves, nor will it absorb crash, recoil, or recurrent damage. However, a substitute will absorb self-inflicted confusion damage and prevent the user from flinching.

If a Pokémon breaks a substitute with Hyper Beam, it will not need to recharge. If a Pokémon breaks a substitute with Explosion or Selfdestruct, it will not faint, though its picture will no longer be visible until it switches out or uses Substitute. If a Pokémon breaks a substitute with a recoil move, it will not take any recoil damage. If a Pokémon breaks a substitute with a draining move, no HP will be restored to it. Note that in each of these cases, the substitute has to be broken, not merely damaged, by the referenced attack.

If the user's current HP is less than 25% (rounded down) of its maximum HP, it will be too weak to make a substitute. If the user's current HP is exactly equal to 25% (rounded down) of its maximum HP, it will faint upon creating the Substitute.

In Stadium, if the user's current HP is exactly equal to 25% (rounded down) of its maximum HP, it will be too weak to create a substitute. If a Pokémon breaks a substitute with Explosion or Selfdestruct, it will faint.